Full-back Mike Brown and centre Ben Te’o are among those who have been left out from Eddie Jones’ England squad to face Wales in Twickenham this weekend.
The exclusion means that pair will miss a final chance to impress the England head coach ahead of the Rugby World Cup as Jones is set to name his final 31-man squad for the tournament on Monday.
Ben Spencer, Alex Dombrandt have also missed out on a place in the matchday squad while Jones has opted not to recall Danny Cipriani either.
However, Jones’ has warned the excluded players to be prepared should another opportunity to impress arise.
“We have worked hard and now move into a four-game preparation phase where the development of the team tactically is paramount. We are grateful for the strong opposition to test our game fitness.
“By the time we fly out on the 8th of September we will be ready to win the Rugby World Cup. We are not there yet but we have four games to get ready.
“Players excluded from being involved in this camp are naturally disappointed but may get another opportunity so they must be ready.“
England will play Wales (11 August) and Ireland (24 August) at Twickenham Stadium and Italy (6 September) in Newcastle as part of the Quilter Internationals as well as an away fixture against Wales (17 August) in Cardiff.
Dan Cole (Leicester Tigers, 85 caps)
Luke Cowan-Dickie (Exeter Chiefs, 11 caps)
Tom Curry (Sale Sharks, 10 caps)
Charlie Ewels (Bath Rugby, 10 caps)
Ellis Genge (Leicester Tigers, 9 caps)
Jamie George (Saracens, 37 caps)
Maro Itoje (Saracens, 27 caps)
George Kruis (Saracens, 32 caps)
Joe Launchbury (Wasps, 58 caps)
Courtney Lawes (Northampton Saints, 71 caps)
Lewis Ludlam (Northampton Saints, uncapped)
Joe Marler (Harlequins, 59 caps)
Kyle Sinckler (Harlequins, 22 caps)
Jack Singleton (Saracens, uncapped)
Sam Underhill (Bath Rugby, 9 caps)
Billy Vunipola (Saracens, 41 caps)
Mako Vunipola (Saracens, 53 caps)
Harry Williams (Exeter Chiefs, 17 caps)
Mark Wilson (Newcastle Falcons / Sale Sharks, 13 caps)
Joe Cokanasiga (Bath Rugby, 4 caps)
Elliot Daly (Saracens, 30 caps)
Owen Farrell (Saracens, 70 caps)
George Ford (Leicester Tigers, 55 caps)
Piers Francis (Northampton Saints, 4 caps)
Willi Heinz (Gloucester Rugby, uncapped)
Jonathan Joseph (Bath Rugby, 40 caps)
Joe Marchant (Harlequins, uncapped)
Jonny May (Leicester Tigers, 45 caps)
Ruaridh McConnochie (Bath Rugby, uncapped)
Henry Slade (Exeter Chiefs, 22 caps)
Manu Tuilagi (Leicester Tigers, 32 caps)
Anthony Watson (Bath Rugby, 33 caps)
Ben Youngs (Leicester Tigers, 85 caps)
Jack Nowell (Exeter Chiefs, 33 caps)